SAP1 — Schizosaccharomyces pombe 972h-

Summary

SPBC947.01 (SAP1, also known as knk1) on chromosome 2 of Schizosaccharomyces pombe encodes a microtubule-severing AAA ATPase (EC 5.6.1.1) with GTPase inhibitor, membrane protein dislocase, and polyubiquitin modification-dependent protein binding activities. The product localises to the ESCRT-IV complex, endosome membrane, mitochondrial outer membrane, and cell tips. SAP1 is associated with microtubule severing and bundling, membrane fission, MVB formation, endosomal transport, centromere separation, mitotic nuclear membrane reassembly, and negative regulation of mitochondrial fission. Phenotypic associations include abnormal chromosome segregation, premature chromosome segregation, decreased meiotic recombination, decreased spore germination, decreased pseudohyphal growth, decreased osmotic stress resistance, decreased radiation resistance, and altered cell cycle progression including S phase arrest and G2/M phase arrest. The gene is also linked to decreased nitrogen source utilisation, decreased pheromone sensitivity, and abnormal spindle morphology.