MAE1 — Schizosaccharomyces pombe 972h-

Summary

SPAC750.08C (MAE1, SpMAE1) on chromosome 1 of Schizosaccharomyces pombe encodes a predicted NAD-dependent malic enzyme with malic enzyme activity, malate dehydrogenase (decarboxylating) activity (NAD+ and NADP+), and oxaloacetate decarboxylase activity, binding NAD and metal ions. The gene is associated with malate metabolism, C4-dicarboxylate and malate transport, ethanol anabolism, malolactic fermentation, carbon catabolite repression, fermentation, pH reduction, secretion, and hypersensitivity. It localises to mitochondria, the mitochondrial lumen, cytosol, nucleus, and hydrogenosome. Phenotypically, MAE1 is linked to decreased anaerobic, fermentative, respiratory, filamentous, and invasive growth; increased protein secretion; decreased gamma ray, freeze-thaw, ionic stress, and chemical resistance; increased killer toxin resistance; increased cold sensitivity and innate thermotolerance; abnormal cell shape, size, budding, and ER morphology; decreased sporulation, autophagy, and mitotic recombination; and altered carbon and nitrogen source utilisation.