MRC1 — Schizosaccharomyces pombe 972h-

Summary

The fission yeast gene mrc1 (SPAC694.06C) on chromosome 1 encodes a claspin-family protein containing a replication checkpoint MRC1 domain. The product acts as a signaling adaptor and binds various DNA structures, including 3'-flap, bubble, D-loop, and Y-form DNA, as well as double-stranded DNA and the APC complex. It also exhibits histone H3-H4 carrier activity. MRC1 is localized to the nucleus, chromatin, and the FPC. The gene is associated with multiple DNA replication and checkpoint processes, including replication fork arrest at tRNA loci, rDNA repeats, and the mating type locus, as well as mitotic G2 and intra-S DNA damage checkpoints, the S-M checkpoint, DNA replication elongation and initiation, and replication-dependent chromatin assembly. Additional associations include trans-autophosphorylation, protein kinase A activation, epigenetic regulation of gene expression, nucleotide metabolism, memory, and rDNA maintenance.