MPH1 — Schizosaccharomyces pombe 972h-

Summary

SPAC20H4.04 (MPH1, fml2) on chromosome 1 of Schizosaccharomyces pombe encodes a DNA/RNA helicase with ATP hydrolase activity, belonging to the FANCM/Mph1-like helicase family. It binds flap-structured DNA and Holliday junctions and exhibits 3'-5' DNA helicase activity. The gene is associated with meiotic strand displacement involved in double-strand break repair via synthesis-dependent strand annealing, negative regulation of strand invasion, and recombinational interstrand cross-link repair. It is linked to MCC assembly, spindle orientation, centromere localization, and Okazaki fragment processing involved in mitotic DNA replication. Phenotypic associations include abnormal cell cycle progression, absent X-ray and UV resistance, decreased lifespan, delayed sporulation, and abnormal mating-type switching and meiotic recombination. The product localizes to the nucleus, nucleolus, cytosol, and the FANCM-MHF complex at sites of double-strand breaks.