REV3 — Saccharomyces cerevisiae S288c

Summary

YPL167C, also known as REV3 in Saccharomyces cerevisiae S288c, encodes a protein with 3'-5' DNA exonuclease activity, DNA-directed DNA polymerase activity, and DNA replication origin binding. It contains a 4Fe-4S cluster and binds zinc, nucleotides, single-stranded DNA, and chromatin. The gene is associated with postreplication repair, bypass DNA synthesis, base-excision repair gap-filling, the DNA damage checkpoint, and response to methyl methanesulfonate. It localizes to the zeta, delta, and alpha DNA polymerase complexes, the nuclear replication fork, and sites of double-strand breaks. Phenotypic associations include increased mutation frequency, decreased viability, and reduced resistance to UV, gamma rays, oxidative stress, and metals, alongside altered competitive fitness and vacuolar morphology.