POL2 — Saccharomyces cerevisiae S288c

Summary

YNL262W (POL2) in Saccharomyces cerevisiae S288c encodes a protein with DNA-directed DNA polymerase activity, single-stranded DNA 3′-5′ exonuclease activity, SUMO binding, and 4Fe-4S cluster, zinc, and nucleotide binding. The protein localises to the DNA polymerase epsilon complex, nuclear replication fork, and telomeric repeat regions. It is associated with nuclear DNA replication, mismatch repair, base-excision repair gap-filling, ribonucleotide excision repair, homologous recombination, sister chromatid cohesion, CMG complex assembly, and leading-strand elongation. The gene is linked to cell cycle progression, including S-phase arrest and delayed progression, as well as increased mutation frequency, decreased spore germination, increased heat sensitivity, decreased competitive fitness, and inviability. It is also associated with oxidative stress and decreased vegetative growth. Studies of related genes in other species link the family to DNA polymerase activity (EC 2.7.7.7) and unaffected pathogenicity.