RAD5 — Saccharomyces cerevisiae S288c

Summary

YLR032W in Saccharomyces cerevisiae S288c encodes a protein with ATP-dependent helicase activity, Holliday junction binding, and ubiquitin ligase activity, containing SNF2-related helicase and RING-type zinc finger domains. The gene is associated with multiple DNA repair pathways, including postreplication repair, homologous recombination, nucleotide excision repair, and mismatch repair, as well as bypass DNA synthesis, replication fork reversal, and telomere maintenance. It localizes to the nucleus, chromatin, and sites of double-strand breaks. Phenotypic associations link YLR032W to decreased resistance to UV, gamma, and X-ray radiation, increased heat sensitivity, reduced oxidative stress tolerance, decreased replicative lifespan, altered telomere length, abnormal chromosome maintenance, and reduced competitive fitness. The gene is also associated with light sensitivity and oxidative stress. Studies of related genes in other species further link these proteins to DNA damage tolerance pathways.