PRP8 — Saccharomyces cerevisiae S288c

Summary

YHR165C (PRP8) in Saccharomyces cerevisiae encodes a protein with metallopeptidase and thiol-dependent deubiquitinase activity, and binds U1, U2, U5, and U6 snRNAs. It is associated with spliceosomal tri-snRNP assembly, mRNA cis-splicing, and spliceosomal conformational changes to generate catalytic conformation. The gene product localizes to the U5 snRNP, Prp19 complex, and catalytic step 2 spliceosome. YHR165C is associated with RNA processing, translation, chromosome condensation, spindle organization, and microtubule assembly. Phenotypically, it is associated with inviability, increased cold and heat sensitivity, increased RNA accumulation, decreased chromosome/plasmid maintenance, and absent sporulation. Studies of related genes in other species link the family to spliceosome function and RNA splicing.