DNA2 — Saccharomyces cerevisiae S288c

Summary

YHR164C in Saccharomyces cerevisiae S288c encodes a protein with 5' flap endonuclease, 5'-3' DNA/RNA helicase, and 5' overhang single-stranded DNA endodeoxyribonuclease activities, also binding 4Fe-4S clusters, chromatin, and single-stranded DNA. The gene is associated with DNA replication, particularly Okazaki fragment processing and lagging strand elongation, as well as telomere maintenance, replication fork reversal, DNA repair upregulation, and the intra-S DNA damage checkpoint. It localises to replication forks, DNA helicase A complexes, nucleolar chromatin, mitochondrial nucleoids, and sites of double-strand breaks. Phenotypic associations include decreased replicative lifespan, increased mutation frequency, cell cycle arrest, delayed S phase progression, decreased vegetative growth, increased heat sensitivity, and altered chemical resistance. Studies of related genes in other species link the family to ATP-dependent helicase and nuclease activities.