DOA4 — Saccharomyces cerevisiae S288c

Summary

YDR069C, also known as DOA4, encodes a metal-dependent deubiquitinase with thiol-dependent deubiquitinase activity, belonging to the ubiquitin carboxyl-terminal hydrolase family. In Saccharomyces cerevisiae, this gene is associated with ubiquitin recycling, free ubiquitin chain depolymerization, vacuolar protein sorting, vacuole fusion, ESCRT III complex assembly, endosome membrane budding, and membrane fusion. It is linked to the response to heat, carbon catabolite repression of transcription, ATP export, proteolysis, lipid metabolism, and receptor recycling. The protein localizes to the late endosome membrane, endosome, proteasome, cellular bud neck, and incipient cellular bud site. Phenotypically, DOA4 is associated with decreased resistance to osmotic, oxidative, and acid stress, decreased replicative and chronological lifespan, abnormal vacuolar transport, decreased endocytosis, and altered utilization of iron and carbon sources.