RPO21 — Saccharomyces cerevisiae S288c

Summary

YDL140C (RPB1) in Saccharomyces cerevisiae S288c encodes the largest subunit of RNA polymerase II, possessing DNA-directed RNA polymerase activity, RNA-dependent RNA polymerase activity, and DNA-binding capacity, and it also functions as a chromatin adaptor. The gene is associated with transcription elongation from RNA polymerase II promoters, transcription initiation from RNA polymerase III promoters, tRNA transcription, mRNA processing, RNA capping, co-transcriptional lncRNA 3' end processing, and nucleotide biosynthesis (UTP, CTP, GTP). It is linked to responses to UV and UV-A, transcription-coupled nucleotide excision repair, sumoylation, methylation, nuclear export, and the DNA damage checkpoint. The protein localizes to RNA polymerase I, II, and III complexes, chromatin, the nucleolus, nucleus, cytosol, and mitochondria. Phenotypic associations include decreased viability, altered cell cycle progression, increased cold and heat sensitivity, reduced vegetative and invasive growth, and altered chemical resistance.