POL3 — Saccharomyces cerevisiae S288c

Summary

YDL102W encodes a B-family DNA polymerase with 3'-5' exonuclease activity, 4Fe-4S cluster binding, and DNA replication origin binding. This nuclear enzyme is associated with DNA replication, including leading and lagging strand elongation, Okazaki fragment processing, and proofreading. It is associated with multiple DNA repair pathways—mismatch repair, base-excision repair, nucleotide-excision repair, and UV-induced excision repair—as well as mitotic recombination and the DNA damage response. The protein localises to replication forks, telomeric regions, and the mitochondrial chromosome. In Saccharomyces cerevisiae, YDL102W is linked to cell cycle progression through S and G2/M phases, meiotic arrest, sporulation defects, increased mutation frequency, elevated mitotic recombination, reduced gamma ray resistance, heat sensitivity, abnormal cellular morphology, and decreased viability. It also shows associations with oxidative stress and altered chemical resistance.