PIF1 — Schizosaccharomyces pombe 972h-

Summary

SPBC887.14C (PIF1/pfh1) in Schizosaccharomyces pombe encodes a Pif1-family DNA helicase with 5'-3' DNA/RNA helicase activity, G4 unwinding activity, DNA reannealing activity, telomerase inhibitor activity, flap-structured DNA binding, 3'-5' exonuclease activity, ATP-dependent protein-DNA complex displacement activity, G-DNA binding, telomeric repeat binding, centromere binding, and ssDNA-dependent ATPase activity. The gene is associated with negative regulation of telomere maintenance via telomerase, DNA amplification, strand displacement, removal of RNA primer in mitotic DNA replication, mitochondrial DNA replication and repair, replication fork reversal, mitotic recombination-dependent replication fork processing, DNA strand elongation, double-strand break repair via break-induced replication, G2 phase, nuclear DNA replication, mating type switching, bypass DNA synthesis, telomere maintenance via recombination, replication fork processing, DNA anabolism, and DNA damage checkpoint. It localizes to the nuclear replisome, rDNA heterochromatin, telomeric repeat region, nuclear replication fork, mitochondrial membrane, site of double-strand breaks, nucleolus, chromatin, mitochondria, and nucleus. Phenotypic associations include altered cell cycle progression, mitotic recombination, sporulation efficiency, thermotolerance, gamma ray resistance, mitochondrial genome maintenance, and chronological lifespan. Related genes in other species link the family to zinc sensitivity, potassium concentration,