PAP1 — Schizosaccharomyces pombe 972h-

Summary

SPBC646.04, also known as PAP1 or pla1 in Schizosaccharomyces pombe 972h- on chromosome 2, encodes a poly(A) polymerase with RNA adenylating enzyme activity (EC 2.7.7.19), binding ATP, RNA, and magnesium. This nuclear protein localizes to cleavage bodies, the Mmi1 nuclear focus, and the mRNA cleavage and polyadenylation specificity factor complex. PAP1 is associated with RNA 3'-end processing, sno(s)RNA 3'-end processing, termination of RNA polymerase II transcription, and RNA surveillance. It is linked to sexual sporulation, spindle pole body separation, and responses to hydrogen peroxide, methylglyoxal, nitrosative stress, and glucose. The gene is associated with ferroptosis, ergosterol metabolism, and reactive oxygen species anabolism. Phenotypic associations include abnormal cell cycle progression, cytokinesis, septum formation, chromosome segregation, cell size, and spore germination, as well as increased virulence and flocculation, and decreased polyploidy.