ADE3 — Schizosaccharomyces pombe 972h-

Summary

SPBC839.16 (ADE3, also known as thf1) on chromosome 2 of Schizosaccharomyces pombe encodes a C1-THF synthase, with methylenetetrahydrofolate dehydrogenase (NAD+ and NADP+) activity, 5,10-methenyl-THF cyclohydrolase activity, and 10-formyl-THF synthetase activity (EC 6.3.4.3, 1.5.1.5, 3.5.4.9). The gene is associated with tetrahydrofolate interconversion, one-carbon metabolism, folate and histidine biosynthesis, purine nucleotide anabolism, and mitotic and meiotic chromosome segregation, kinetochore assembly, spindle checkpoint function, and microtubule dynamics. It localizes to the nucleus, cytosol, and mitochondria. Phenotypically, ADE3 is linked to altered cell cycle progression, increased critical cell size at the G1 checkpoint, abnormal mitochondrial genome maintenance and transport, decreased mitophagy and pexophagy, altered nutrient utilization, increased fermentative metabolism, and changes in acquired thermotolerance and sporulation efficiency.