POL3 — Schizosaccharomyces pombe 972h-

Summary

POL3 (cdc6) on chromosome 2 of Schizosaccharomyces pombe encodes a B-family DNA-directed DNA polymerase with 3'-5' exonuclease activity, 4Fe-4S cluster binding and DNA replication origin binding. The product is a catalytic subunit of the delta, alpha and zeta DNA polymerase complexes and localises to the nucleus, chromatin, replication forks, telomeric regions and sites of double-strand breaks. POL3 is associated with DNA replication initiation, leading and lagging strand elongation, Okazaki fragment RNA removal, proofreading, base-excision and nucleotide-excision repair gap-filling, and pre-RC assembly. It is linked to meiotic and mitotic S phase, cell cycle progression through G1/S and G2/M transitions, and to phenotypes including delayed vegetative growth, abnormal mating response, premature chromosome segregation, decreased gamma ray resistance, and abnormal meiotic recombination and sporulation timing. Studies of related genes in other species link the family to meiotic arrest.