ARK1 — Schizosaccharomyces pombe 972h-

Summary

ARK1 (alg10) of Schizosaccharomyces pombe 972h- on chromosome 1 encodes a dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase (EC 2.4.1.256) with transglycosidase activity, localising to the lumenal side of the endoplasmic reticulum membrane. The gene is associated with oligosaccharide-PP-dolichol assembly and glycoprotein anabolism, as well as numerous cell cycle and chromosome dynamics processes including regulation of chromosome segregation, centrosome duplication, kinetochore assembly, sister chromatid segregation, telophase, and chromosome condensation. It is linked to TORC2 signaling, microtubule shortening, meiosis II, and cellular homeostasis. Phenotypic associations include delayed G2 phase cell cycle progression, S phase arrest, decreased cytokinesis and endocytosis rates, increased polyploidy, abnormal filamentous and pseudohyphal growth, decreased mating type switching, and altered pheromone sensitivity. Related genes in other species link the family to cell membrane stability, protein content, and phosphate content.