RAD4 — Schizosaccharomyces pombe 972h-

Summary

SPAC12B10.12C (RAD4, rhp41) on chromosome 1 of Schizosaccharomyces pombe encodes a DNA repair protein belonging to the Rad4 subfamily, characterised by beta-hairpin domains and a transglutaminase-like fold. The product binds damaged and single-stranded DNA and is a component of the XPC and NEF2 complexes. The gene is associated with multiple DNA repair pathways including nucleotide excision repair (NER), transcription-coupled NER, UV-damage excision repair, base excision repair, mismatch repair, and double-strand break repair. It is linked to cell cycle checkpoint control, replication fork arrest, pre-replicative complex assembly, and response to radiation and hydroxyurea. Phenotypic associations include altered UV, gamma ray, and X-ray resistance, increased mutation frequency, abnormal chromosome segregation, increased polyploidy, decreased colony sectoring, and abnormal telomere length, autophagy, and vacuolar transport. Studies of related genes in other species link the family to oxidative stress response.