SEN1 — Saccharomyces cerevisiae S288c

Summary

YLR430W, also known as SEN1, encodes a 5'-3' RNA helicase with ATP hydrolase activity, dsDNA-dependent ATPase activity, and 5'-3' DNA/RNA helicase activity, and binds RNA, ssDNA, and transcription termination site sequence-specific DNA. This gene is associated with RNA 3'-end formation by RNA polymerase II, RNA polymerase III transcription termination, transcriptional attenuation, RNA processing, tRNA metabolism, DNA damage response, homologous recombination, mitotic DNA replication lagging strand elongation, and regulation of mRNA stability in response to oxidative stress. It is linked to leaf senescence, nodulation, nitrogen fixation, intercellular transport, and regulation of gene expression. The protein localizes to the nucleus, nucleolar chromatin, nuclear body, nuclear replication fork, site of double-strand break, DNA helicase A complex, cytoplasm, and cytosol. SEN1 is associated with increased apoptosis, decreased chronological lifespan, decreased respiratory growth, increased cold and heat sensitivity, decreased UV and oxidative stress resistance, decreased vegetative growth rate, abnormal vacuolar and lipid particle morphology, and decreased mitochondrial genome maintenance. Studies of related genes in other species link the family to RNA helicase activity (EC 3.6.4.13).