MPH1 — Saccharomyces cerevisiae S288c

Summary

YIR002C in Saccharomyces cerevisiae S288c encodes a DNA/RNA helicase with ATP hydrolase activity that binds ATP, flap-structured DNA, and Holliday junctions, and exhibits 3'-5' DNA helicase activity. The protein localises to the nucleus and is associated with multiple DNA repair pathways, including recombinational repair, base excision repair, postreplication repair, and double-strand break repair via synthesis-dependent strand annealing. It is linked to DNA replication Okazaki fragment processing, telomere maintenance and assembly, sister chromatid cohesion, spindle assembly, the DNA damage checkpoint, and MCC assembly. The gene is associated with traits including protein content and oxidative stress, and phenotypes such as decreased viability, increased mutation frequency, decreased UV resistance, increased chronological lifespan, decreased competitive fitness, and altered metal and chemical resistance. Studies of related genes in other species link the family to DNA/RNA helicase activity (EC 3.6.4.12).