ECM32 — Saccharomyces cerevisiae S288c

Summary

YER176W, also known as UPF1 or ECM32 on chromosome V of Saccharomyces cerevisiae S288c, encodes a protein with 5'-3' RNA helicase activity, dsDNA-dependent ATPase activity, 5'-3' DNA/RNA helicase activity, transcription termination site sequence-specific DNA binding, lncRNA binding, 5'-3' DNA helicase activity, RNA helicase activity, ssDNA binding, RNA binding, ATP hydrolase activity, zinc binding, and ATP binding. The gene is associated with RNA breakdown, mRNA breakdown, gene silencing by miRNA, viral assembly, arginine anabolism, RNA processing, regulation of mRNA stability involved in response to oxidative stress, mitotic DNA replication lagging strand elongation, tRNA metabolism, homologous recombination, regulation of mRNA stability, RNA polymerase III transcription termination, thiamin metabolism, sensitization, RNA 3' end processing, nuclear mRNA breakdown, regulation of DNA repair, and RNAi-like chromatin silencing. Localization evidence connects the product to the DNA helicase A complex, RNA-directed RNA polymerase complex, RDRC, nucleolar chromatin, nuclear body, nuclear replication fork, site of DSB, chromatin, and cytoplasm. Phenotypic associations include increased invasive growth, decreased starvation resistance, and reduced competitive fitness. Studies of related genes in other species link the family to RNA helicase and ATPase activities.