RAD3 — Saccharomyces cerevisiae S288c

Summary

YER171W in Saccharomyces cerevisiae S288c encodes a protein with 5'-3' DNA helicase activity, DNA helicase activity, DNA binding, ATP binding, Fe/S cluster binding, damaged DNA binding, GTF2 activity, ATP hydrolase activity, and metal binding, and is a component of the core and holo TFIIH complexes as well as the NEF3 complex. The gene is associated with transcription by RNA polymerase II, mitotic recombination, gene conversion, upregulation of mitotic recombination, regulation of mismatch repair, meiotic heteroduplex formation, RNA anabolism, nucleotide excision repair, DNA-dependent DNA replication maintenance of fidelity, double-strand break repair, DNA anabolism, nucleobase-containing compound metabolic process, gastrulation, heteroduplex formation, response to radiation, regulation of phosphorylation, microtubule destabilization, and homologous recombination. It has been linked to respiration rate and oxidative stress. Phenotypic associations include increased mitotic recombination and mutation frequency, decreased resistance to ionizing radiation and starvation, altered UV resistance, abnormal S-phase cell cycle progression, increased nucleolus size, decreased chromosome maintenance, heat sensitivity, altered chemical resistance, inviability, and haploinsufficiency. Studies of related genes in other species connect the family to DNA helicase activity (EC 5.6.2.3).