RAD4 — Saccharomyces cerevisiae S288c

Summary

YER162C (RAD4) in Saccharomyces cerevisiae S288c on chromosome V encodes a protein that belongs to the Rad4/PNGase transglutaminase-like superfamily and is associated with damaged DNA binding and ssDNA binding. The gene product is associated with multiple DNA repair pathways, including NER, base excision repair (BER), mismatch repair (MMR), homologous recombination, and gene conversion, as well as meiotic heteroduplex formation and the DNA damage checkpoint. RAD4 has been linked to chromatin remodeling, proteolysis, methylation, and the multivesicular body sorting pathway. It localizes to the nucleus, nucleolus, cytoplasm, mitotic spindle, and the XPC and NEF2 complexes. Phenotypic associations include decreased UV resistance, increased mitotic recombination and mutation frequency, reduced chronological lifespan, altered metal and chemical resistance, and decreased competitive fitness. The gene is also associated with oxidative stress.