DO — Magnaporthe oryzae 70-15

Summary

MGG_00865 (MoDO) encodes a protein with branched 1,3-beta-D-glucan synthase activity, callose synthase activity, and alpha-1,3-glucan synthase activity (EC 2.4.1.34), placing it in the FKS1-like and glycosyltransferase 48 families. In Magnaporthe oryzae, MoDO is associated with beta-glucan and ascospore wall 1,3-beta-glucan biosynthesis, primary and secondary cell septum biogenesis, mitotic division septum assembly, cell wall biogenesis and organization, autophagy, host invasion, and regulation of cell polarity and shape. The protein localizes to the 1,3-beta-glucan synthase complex, primary cell septum, spore wall, cleavage furrow, growing cell tips, perinuclear ER, and the mitotic actomyosin contractile ring. Associations with decreased spore germination rate, abnormal shmoo formation, decreased adhesion, and altered cell cycle progression have been noted. Studies of related genes in other species link the family to reduced virulence, sporogenesis, beta-glucan content, and oxidative stress response.