CHS1 — Verticillium dahliae

Summary

VDAG_10179 (CHS1, VdCHS1) in Verticillium dahliae encodes a chitin synthase with chitin synthase activity (EC 2.4.1.16), containing chitin_synth_N and chitin_synth domains. The gene is associated with chitin anabolism, cell wall organization, septum positioning, mitotic actomyosin contractile ring contraction, and conidia formation. The encoded protein localizes to the plasma membrane, cell periphery, cellular bud neck, and cross wall. Associations with numerous phenotypes include abnormal chitin deposition, septum formation, spore wall formation, bipolar budding, shmoo formation, cell fusion, adhesion, vesicle distribution, and filamentous growth; decreased flocculation, cell death, and axial budding; increased pheromone sensitivity, respiratory and fermentative metabolism, and survival rate in stationary phase; and absent budding, cell fusion, septum formation, and filamentous growth. The gene is linked to vascular wilt in cotton and Arabidopsis, and to polysaccharide content through family-level evidence.