AGO1 — Verticillium dahliae

Summary

VDAG_10484, designated VdAGO1 (also known as AGO1, SMS-2), encodes an Argonaute-family protein in Verticillium dahliae (chromosome DS572735). The product possesses Piwi, PAZ, and Mid domains and has endoribonuclease activity, siRNA binding, and ssRNA binding. VdAGO1 is associated with RNA interference-mediated gene silencing, including chromatin silencing at centromeres and telomeres, siRNA and priRNA 3′-end processing, vegetative phase change, maintenance of G0 phase, and interaction with the host. The protein localises to the RITS and ARC complexes, heterochromatin (including centric, subtelomeric, and mating-type region), the cytoplasm, and the nucleus. Phenotypic associations include abnormal sporulation, decreased UV and oxidative stress resistance, increased heat sensitivity, altered competitive fitness, and increased replicative and chronological lifespan.