ASN1 — Verticillium dahliae

Summary

VdASN1 (VDAG_00271) in Verticillium dahliae encodes an asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4), a member of the AsnB_N and Asn_synthase domain families, with ATP-binding and hydrolase activities. It is associated with L-asparagine biosynthesis and with posttranscriptional regulation of gene expression. The protein localises to both the cytosol and nucleus. Associations with a wide range of phenotypes have been recorded, including decreased replicative lifespan, increased cold sensitivity, altered cell cycle progression (including G1 arrest and metaphase arrest), abnormal chromosome segregation and spindle morphology, increased invasive growth, decreased acquired and innate thermotolerance, increased colony sectoring, and abnormal RNA accumulation and localisation. The gene is also linked to prion inheritance and loss phenotypes, increased fermentative metabolism, decreased hydrostatic pressure resistance, and abnormal telomere length and nucleolus size.