AD-7 — Verticillium dahliae

Summary

VDAG_00128 (VdAD-7) in Verticillium dahliae encodes an amidophosphoribosyltransferase (EC 2.4.2.14), a PurF family enzyme with GATase and PRTase domains. The gene is associated with adenine metabolism, purine base and nucleotide anabolism, and de novo IMP biosynthesis, and its product localises to the cytoplasm. VdAD-7 is associated with abnormal colony color and appearance, increased sporulation efficiency and filamentous growth, increased biofilm formation, decreased cold sensitivity, increased rate of fermentative metabolism, and absent autophagy, absent late nucleophagy, decreased mitophagy, decreased reticulophagy, decreased vacuolar transport, and absent utilization of nitrogen source and decreased utilization of phosphorus source. It is also associated with increased G1 phase duration and increased critical cell size at start, and abnormal nuclear, peroxisomal, and endoplasmic reticulum morphology.