RAD1 — Schizosaccharomyces pombe 972h-

Summary

SPCC970.01 (RAD1, also known as rad16) on chromosome 3 of Schizosaccharomyces pombe encodes a protein with an ERCC4/XPF nuclease domain and 3' overhang single-stranded DNA endonuclease activity. The gene is associated with double-strand break repair via single-strand annealing, resolution of recombination intermediates, DNA integrity checkpoint, and response to radiation. It is linked to meiotic prophase I, spermatogenesis, telomere capping, replication fork arrest, and interstrand crosslink repair. Phenotypic associations include absent X-ray resistance, abnormal sporulation efficiency, delayed exponential growth, decreased lifespan, absent mating-type switching, arrested meiosis, premature chromosome segregation, and increased necrotic cell death. The product localizes to the nucleus, cytoplasm, mitotic spindle pole body, and NEF1 complex. Studies of related genes in other species link the family to cell membrane stability and oxidative stress response.