SEN1 — Schizosaccharomyces pombe 972h-

Summary

SPBC29A10.10C, also known as SEN1 or dbl8 in Schizosaccharomyces pombe 972h- on chromosome 2, encodes an uncharacterized ATP-dependent helicase with a Sen1 N-terminal domain and a DNA2/NAM7-like architecture. The gene product exhibits 5'-3' RNA helicase activity, 5' flap endonuclease activity, and dsDNA-dependent ATPase activity, and it binds lncRNA and 4Fe-4S clusters. SEN1 is associated with R-loop processing, Okazaki fragment processing during mitotic DNA replication, replication fork reversal, and nonsense-mediated mRNA decay. It localizes to the replication fork, the RNA-directed RNA polymerase complex, and nucleolar chromatin. Phenotypic associations link SEN1 to decreased cell cycle progression through G2/M and G1/S transitions, delayed chromosome segregation, increased gamma ray resistance, increased thermotolerance, absent biofilm formation, and abnormal telomere length. Related genes in other species connect the family to RNA 3'-end processing and transcription termination.