SAP1 — Schizosaccharomyces pombe 972h-

Summary

SPAC328.04 (SAP1) on chromosome 1 of Schizosaccharomyces pombe encodes an uncharacterized AAA domain-containing protein with microtubule-severing AAA ATPase activity. The gene product is associated with multiple molecular functions including GTPase inhibitor activity, membrane protein dislocase activity, preribosome binding, ATP-dependent protein disaggregase activity, polyubiquitin modification-dependent protein binding, ubiquitin ligase binding, protein homodimerization activity, and ATP hydrolase activity. SAP1 is linked to biological processes such as cell fate determination, centromere separation, negative regulation of mitochondrial fission, extraction of mislocalized protein from mitochondrial outer membrane, microtubule severing, MVB formation, membrane fission, mitotic nuclear membrane reassembly, microtubule bundling, endosome membrane budding, protein-Golgi retention, activation of cellular response to hypoxic stress, nuclear protein quality control by the ubiquitin-proteasome system, replication fork arrest, the INMAD pathway, and endosomal transport. It localizes to the ESCRT-IV complex, Vms1-Cdc48-Npl4 complex, medial cortex septin ring, Hrd1p ubiquitin ligase complex, RQC complex, p97-Ufd1-Npl4 complex, and endosome membrane. Phenotypically, SAP1 is associated with abnormal chromosome segregation, decreased meiotic recombination, altered cell cycle progression, decreased radiation resistance, and decreased osmotic stress