RAD54 — Schizosaccharomyces pombe 972h-

Summary

SPAC15A10.03C (rad54) in Schizosaccharomyces pombe encodes a protein with Rad54_N, SNF2_RAD54_helicase_repair, SNF2/RAD54-like_C, SNF2-like_sf, SNF2_N, Helicase_ATP-bd, Helicase_C, and P-loop_NTPase domains, and has histone octamer slider, nucleosome array spacer, DNA translocase, TBP-class protein binding, ATP-dependent chromatin remodeler, centromere binding, histone carrier, DNA helicase, nucleosome binding, helicase, chromatin adaptor, GTF2, and histone binding activities. The gene is associated with double-strand break repair via synthesis-dependent strand annealing, meiotic gene conversion, regulation of mitotic recombination, meiotic prophase I, pachytene, replication fork arrest, nucleosome disassembly and organization, V-J joining, response to UV and UV-A, pre-RC assembly, and regulation of RNA polymerase II transcription preinitiation complex assembly. It localizes to the SWI-SNF and PBAF complexes, CHRAC, nuclear microtubules, CENP-A-containing chromatin, and sites of double-strand breaks. Phenotypic associations include absent mitotic recombination, delayed meiotic recombination, arrested meiosis, abnormal sporulation, increased necrotic cell death, decreased lifespan, premature chromosome segregation, and increased thermotolerance.