TFP1 — Neurospora crassa

Summary

NCU01207 (TFP1, vma-1) in Neurospora crassa encodes a V-type proton ATPase catalytic subunit A, a V1 complex ATPase with proton-transporting and ATP hydrolase activities, and also possesses endonuclease activity. The gene is associated with vacuolar acidification, proton transmembrane transport, and cellular chemical homeostasis, as well as ATP metabolism and proton motive force-driven mitochondrial ATP synthesis. It is linked to hyphal growth, cell-cell fusion, endocytosis, and the cellular response to oxidative stress. Phenotypic associations include abnormal vacuolar morphology and distribution, altered filamentous growth, decreased sporulation efficiency, increased adhesion, and absent alkaline pH resistance. The gene is also connected to altered protein transport, endocytosis, and respiratory metabolism, as well as decreased cell death and ribophagy. Studies of related genes in other species link the family to sensitivity to zinc, calcium, and acid, and to oxidative stress response.