PLC1 — Neurospora crassa

Summary

NCU01266 (NcPLC1) on Neurospora crassa chromosome 5 encodes a phosphoinositide phospholipase C (EC 3.1.4.11) with phosphatidylinositol-4,5-bisphosphate phospholipase C activity and calcium ion binding, containing PH, C2, EF-hand and PI-PLC family domains. The gene is associated with phosphoinositide-mediated signaling, calcium signaling, inositol phosphate anabolism, lipolysis, and cytosolic calcium release, and localises to the cytoplasm, cytosol and nucleus. NcPLC1 is linked to carotenoid content and numerous phenotypes including absent alkaline pH resistance, absent hyperosmotic stress resistance, absent pheromone sensitivity, absent adhesion, absent cell cycle progression, premature anaphase progression, increased polyploidy rate, abnormal nucleophagy, abnormal vacuolar distribution, abnormal nuclear export, decreased sulfur and nitrogen utilisation, and increased nutrient uptake. Studies of related genes in other species connect the family to zinc and calcium sensitivity, phosphate content, and increased ionic stress resistance.