SKP1 — Fusarium graminearum PH-1

Summary

FgSKP1 (FGRAMPH1_01G23503) on chromosome 4 of Fusarium graminearum PH-1 encodes a SKP1-like protein that contains a Skp1 component dimerisation domain and binds cullin family proteins, with associated ligase activity. The gene is associated with protein ubiquitination and ubiquitin-dependent proteolysis, including SCF-dependent proteasomal breakdown, as well as regulation of DNA recombination, resolution of meiotic recombination intermediates, mitotic cell cycle, sexual reproduction, and mitotic nuclear membrane biogenesis. Associations with phenotypes include decreased cell cycle progression in M phase, premature anaphase entry, abnormal bipolar budding, decreased flocculation and adhesion, altered pseudohyphal growth, delayed sporulation, and increased mating efficiency. The protein localises to the cytoplasm, nucleus, ssDNA-dependent ATP-dependent DNA helicase complex, and RAVE complex. Studies of related genes in yeast link the SKP1 family to the nuclear SCF complex and, through sequence similarity, to loss of pathogenicity in other fungi.