CLM2 — Neurospora crassa

Summary

NCU10344 (CLM2, NcCLM2) on Neurospora crassa chromosome 6 encodes a cytochrome P450 98A3 with monooxygenase activity, heme and iron binding, and oxidoreductase activity acting on paired donors with oxygen incorporation. The gene is associated with ergosterol, sterol, ceramide, phospholipid, antibiotic, mycotoxin, and terpenoid anabolism, as well as sterol homeostasis and hypersensitivity. It is linked to membrane localisation. Phenotypic associations include decreased micronucleophagy, nucleophagy, ribophagy, and shmoo formation; absent hyperosmotic stress resistance, cytokinesis, and chemical compound accumulation; abnormal plasma membrane morphology, cell cycle progression through G2/M, entry into G0, and S phase; increased cell death, fermentative metabolism, and lifespan; arrested vegetative and exponential growth; and decreased invasive growth, nutrient uptake, vacuolar transport, and utilization of iron, phosphorus, and carbon sources. Studies of related genes in other species link the family to ergosterol biosynthesis and protein content.