POF1 — Neurospora crassa

Summary

NcPOF1 (NCU08563) on Neurospora crassa chromosome 3 encodes a protein with F-box and WD40-repeat domains that acts as a ubiquitin ligase substrate adaptor and molecular sensor, with ubiquitin-binding and identical protein-binding activities. The gene is associated with regulation of sulfur metabolism, SCF-dependent proteasomal ubiquitin-dependent protein breakdown, and responses to arsenic and cadmium. It has been linked to cell cycle progression, particularly G1/S and G2/M phase transitions, as well as nuclear localization, SCF complex assembly, and transcription regulation by RNA polymerase II. Phenotypic associations include altered sulfur source utilization, cell cycle arrest at multiple phases, decreased pheromone sensitivity, abnormal chronological lifespan, and reduced nuclear fusion during mating. Studies of related genes in other species connect the family to cell cycle control and sulfur metabolism regulation.