NIT2 — Neurospora crassa

Summary

NCU05757 (NIT2) on Neurospora crassa chromosome 7 encodes a nitrilase with deaminated glutathione amidase, bis(5'-adenosyl)-triphosphatase, and ω-amidase activities, belonging to the C-N hydrolase superfamily. It is associated with nitrogen catabolite repression, nitrate assimilation, nitrogen utilization, glutamine metabolism, glycogen metabolism, oxaloacetate metabolism, gibberellin biosynthesis, siderophore anabolism, cellular detoxification, homologous recombination, hyphal growth, nuclear import, and iron transport. The gene product localizes to mitochondria, cytoplasm, cytosol, and nucleus. Phenotypic associations include decreased competitive fitness, absent respiratory growth, increased protein/peptide accumulation, decreased toxin resistance, abnormal and increased chemical compound accumulation, and increased resistance to chemicals. Related genes in other species link the family to hydrolase activity on carbon-nitrogen bonds.