MOT2 — Neurospora crassa

Summary

NCU04073 (MOT2, NcMOT2) on Neurospora crassa chromosome 6 encodes a protein with ubiquitin-protein transferase activity and RNA binding, containing a NOT4_mRING-HC-C4C4 domain and an RRM domain. It is a component of the CCR4-NOT complex and localises to both the nucleus and cytosol. The gene is associated with positive regulation of RNA metabolism and transcription elongation by RNA polymerase II, as well as negative regulation of nuclear mRNA surveillance of meiosis-specific transcripts, mRNA poly(A) tail shortening, protein ubiquitination, and RNA-dependent gene silencing. Phenotypic associations include absent exponential-phase growth, delayed exit from G0 stationary phase, increased carbon source utilisation, decreased sulfur source utilisation, abnormal cell cycle progression in M and S phases, increased hydrostatic pressure resistance, and abnormal pseudohyphal growth, vesicle distribution, and nuclear export. Related genes in other species are linked to oxidative stress response and acid sensitivity.