PRP8 — Fusarium graminearum PH-1

Summary

FgPRP8 (PRP8, SPP42) on chromosome 1 of Fusarium graminearum PH-1 encodes a protein with multiple Prp8 domains, including U5 and U6 snRNA-binding regions, an RNase H-like fold, and a JAMM/MPN+ metallopeptidase domain. The product binds U1, U2, U5 and U6 snRNAs, acts as a thiol-dependent deubiquitinase, and is associated with pre-mRNA intron binding, spliceosomal tri-snRNP assembly, spliceosomal conformational changes to generate catalytic conformation, and meiosis. It localises to U5 snRNP, the Prp19 complex, and the U4/U6.U5 snRNP complex. Phenotypic associations link FgPRP8 to abnormal spindle morphology, increased nuclear size, altered cell cycle progression (including metaphase arrest and premature telophase), abnormal bipolar budding, absent mating-type switching, increased pheromone sensitivity, increased alkaline pH resistance, and increased starvation resistance. The gene is also associated with absent endocytosis, absent pexophagy, and increased meiotic recombination.