GLO3 — Aspergillus nidulans FGSC A4

Summary

ANIA_06033 (GLO3, AnGLO3) on chromosome 1 of Aspergillus nidulans FGSC A4 encodes a putative ARF GTPase activator (Glo3) with an ArfGAP domain, zinc-binding and GAP activity, and polyubiquitin modification-dependent protein binding. The gene is associated with COPI coating of Golgi vesicles, cis-Golgi to rough ER transport, intra-Golgi and ER-to-Golgi transport, endocytosis, membrane fusion, and cell growth mode switching from monopolar to bipolar. It localises to the Golgi membrane, coatomer, clathrin-coated vesicles, cell tip, cell cortex of the cell tip, cell division site, and actin patch. Phenotypic associations include abnormal Golgi morphology, absent spore germination, increased protein transport rate, increased thermotolerance, increased freeze-thaw resistance, decreased cell death, arrested mitotic cell cycle, delayed metaphase-anaphase transition, and absent shmoo formation. Studies of related genes in other species link the family to acid sensitivity and oxidative stress response.