RPB1 — Verticillium dahliae

Summary

VdRPB1 (VDAG_06114) in Verticillium dahliae encodes a protein with RNA polymerase Rpb1 domains and a eukaryotic RNA polymerase II repeat, and is annotated as a DNA-directed RNA polymerase (EC 2.7.7.6) with RNA-dependent RNA polymerase activity, DNA binding, and chromatin adaptor functions. This gene is associated with transcription by all three RNA polymerases, including mRNA, tRNA, and rRNA transcription, as well as mRNA capping, co-transcriptional lncRNA processing, and intracellular phosphate homeostasis. It localizes to the nucleus, nucleolus, chromatin, and RNA polymerase I, II, and III complexes. Phenotypic associations link VdRPB1 to increased virulence, altered cell cycle progression (including G1/S and G2/M transitions), increased sporulation, decreased budding, abnormal mitophagy, increased transposable element transposition, and decreased replicative lifespan. Studies of related genes in other species connect the family to oxidative stress response and acid sensitivity.