DNF1 — Verticillium dahliae

Summary

VDAG_05069, designated VdDNF1 (also known as DNF1, VdDNF3, DNF3) in Verticillium dahliae on chromosome DS572703, encodes a phospholipid-transporting ATPase (EC 7.6.2.1) with glycoceramide, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine flippase and floppase activities, along with ATP hydrolase activity and phosphatidylinositol-4-phosphate binding. The gene is associated with phospholipid, glycolipid, and ceramide translocation, endocytic recycling, post-Golgi transport, secretion, endocytosis, vesicle transport, sporulation, fermentation, and establishment or maintenance of cell polarity. It localizes to the trans-Golgi network membrane, endosome membrane, Golgi membrane, division septum, and the Cdc50p-Drs2p and APLT complexes. Associations with cell cycle progression, pheromone sensitivity, shmoo formation, chitin deposition, and alkaline pH resistance have been noted, while studies of related genes in other species link the family to reduced virulence.