PRS — Verticillium dahliae

Summary

VdPRS (VDAG_02992) in Verticillium dahliae encodes a prolyl-tRNA synthetase with proline-tRNA ligase activity and ATP binding, and contains a prefoldin alpha-like domain. The gene is associated with prolyl-tRNA aminoacylation, protein folding, RNA polymerase I, II and III assembly, mitochondrial translation, cell wall modification, lipogenesis, metabolic burst, and virus-induced PTGS. Its product localises to the cytoplasm, nucleus, mitochondria, mitochondrial lumen, prefoldin complex, cell tip, and cell division site. Phenotypic associations include increased filamentous growth, decreased vegetative growth and competitive fitness, increased heat sensitivity, abnormal RNA localisation and nucleolar morphology, enlarged nucleolus, haploinsufficiency, inviability, and decreased chemical resistance. Studies of related genes in other species link the family to abnormal mitochondrial genome maintenance, altered stress and metal resistance, and decreased respiratory growth.