TI — Verticillium dahliae

Summary

VDAG_03232, designated VdTI, encodes the dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3, an asparagine N-glycosyltransferase (EC 2.4.99.18) that binds metal ions and is a core component of the OST complex in the endoplasmic reticulum membrane. In Verticillium dahliae, VdTI is associated with protein N-linked glycosylation, glycoprotein anabolism, secretion, hyphal growth, germ tube formation, sporulation, and verticillium wilt. The gene is linked to numerous cellular phenotypes including abnormal cell wall morphology, increased chitin deposition, altered protein transport and secretion, decreased exponential-phase growth, increased apoptosis, decreased thermotolerance, and abnormal endomembrane system morphology. VdTI also associates with germination ratio and has been connected to Nicotiana benthamiana. Studies of related genes in other species link it to additional glycosylation-related genes such as ALG5, ALG3, and WBP1.