DNF1 — Verticillium dahliae

Summary

VdDNF1 (VDAG_01765) in Verticillium dahliae encodes a P-type ATPase with phospholipid flippase activity, including phosphatidylserine, phosphatidylcholine, and phosphatidylethanolamine flippase activities, as well as lysophosphatidylserine and glycoceramide flippase activities, and is annotated with cation-transporting ATPase activity. The gene is associated with phospholipid translocation, ceramide and glycolipid translocation, endocytic recycling, post-Golgi transport, sporulation, secretion, endocytosis, and cellular calcium ion homeostasis. It localizes to the trans-Golgi network membrane, endosome membrane, Golgi membrane, and the Cdc50p-Drs2p complex. Phenotypic associations include abnormal septum formation, altered cell cycle progression, abnormal pheromone sensitivity and shmoo formation, increased alkaline pH resistance, decreased chitin deposition, and altered endocytosis. Related genes in other species are linked to reduced virulence.