NIAD — Neurospora crassa

Summary

NCU05298 (NIAD, nit-3) on Neurospora crassa chromosome 4 encodes a protein with nitrate reductase (NADPH) activity (EC 1.7.1.3), sulfite oxidase activity, moco binding, FAD binding, heme binding, and cytochrome-b5 reductase activity acting on NAD(P)H. The protein contains multiple oxidoreductase domains. This gene is associated with nitrate assimilation, nitrate import, regulation of nitrate assimilation, nitrogen utilization, nitrogen catabolite repression, anaerobic respiration, nitric oxide anabolism, and catabolite repression. It is also linked to gibberellin biosynthesis, sulfur metabolism, regeneration, and protein histidyl modification to diphthamide. The gene product localizes to the mitochondrial outer membrane and plasmalemma. Associations with nitrate transport, nitrate uptake, and molybdenum content have been recorded. Studies of related genes in other species link the family to NADH dehydrogenase activity.