ACO1 — Magnaporthe oryzae 70-15

Summary

MoACO1 (MGG_03521) on chromosome 4 of Magnaporthe oryzae 70-15 encodes a protein with aconitase and homoaconitate hydratase activities, also annotated as a 3-isopropylmalate dehydratase, and binds 4Fe-4S clusters, dsDNA, ssDNA, and mRNA, with structural ribosome constituent properties. The gene is associated with the TCA cycle, electron transport chain, ATP anabolism, ubiquinone anabolism, L-leucine anabolism, CoA biosynthesis, ferroptosis, mitophagy, autophagy, cell death, and perturbation of host immune response. It localises to mitochondria, mitochondrial inner membrane, nucleoid, large ribosomal subunit, cytoplasm, nucleus, and fungal biofilm matrix. Phenotypic associations include abnormal colony colour and shape, decreased colony size, increased cell death, altered respiratory and fermentative metabolism, increased mitochondrial rho- mutation frequency, and absent mitochondrial genome maintenance. Studies of related genes in other species link the family to calcium sensitivity.