KSR1 — Magnaporthe oryzae 70-15

Summary

MoKSR1 (MGG_00362) on chromosome 5 of Magnaporthe oryzae 70-15 encodes a 3-dehydrosphinganine reductase, a KDSR-like short-chain dehydrogenase/reductase with NADPH binding activity (EC 1.1.1.102). The gene is associated with sphingolipid and GPI anchor biosynthesis, 3-keto-sphinganine metabolism, cell development, and lipid homeostasis. Its product localises to the endoplasmic reticulum membrane and adiposomes. Phenotypic associations link MoKSR1 to decreased stress resistance, including hyperosmotic, acid pH, heat, and oxidative stress responses, as well as altered autophagy, protein transport and modification, vacuolar morphology, biofilm formation, and competitive fitness. The gene is also associated with decreased vegetative growth, nitrogen utilisation, and toxin resistance, alongside increased chemical compound accumulation and oxidative stress resistance. Studies of related genes in other species link the family to inviability and increased toxin resistance.