PRP8 — Aspergillus fumigatus

Summary

AFU2G03030 (PRP8) in Aspergillus fumigatus encodes a pre-mRNA splicing factor (Prp8) that contains multiple domains including Prp8_U5-snRNA-bd, RNaseH-like, and JAMM/MPN+ domains, and is associated with endonuclease, metallopeptidase, thiol-dependent deubiquitinase, and DNA binding activities. The gene is associated with spliceosomal functions including U1, U2, U5, and U6 snRNA binding, pre-mRNA intron binding, second spliceosomal transesterification activity, and protein splicing. It localizes to U5 snRNP, U4/U6.U5 tri-snRNP, and catalytic step 2 spliceosome complexes. PRP8 is linked to biological processes including mRNA splicing, spliceosomal tri-snRNP assembly, biofilm formation, morphogenesis, amino acid anabolism, and inflammatory and immune responses. Phenotypic associations include abnormal spindle morphology, increased nucleus size, altered cell cycle progression, increased starvation resistance, and abnormal mating and budding patterns. Studies of related genes in other species link the family to spliceosome assembly factors CWC21 and HUB1.